Amakuru

Imiyoboro ikoreshwa cyane muri laboratoire kugirango yimure mililitiro yamazi, kuva byibuze ml 1 kugeza kuri ml 50. Ibyatsi birashobora gutabwa muri plastiki sterile cyangwa bigakoreshwa mubirahuri byikora. Imiyoboro yombi ikoresha umuyoboro wo kwifuza no kwirukana amazi. Ingano zitandukanye za pipeti zikoreshwa mubushakashatsi butandukanye hamwe na pipeti imwe. Kurugero, imiyoboro ningirakamaro mu kuvanga ibisubizo byimiti cyangwa guhagarika selile, guhererekanya amazi hagati yabyo bitandukanye, cyangwa reagent ya plaque mubucucike butandukanye. Mugihe cyose hitabwa cyane kubunini bwamazi yifuzwa kandi akirukanwa, imiyoboro irashobora kuba igikoresho cyingirakamaro muri laboratoire yo kohereza neza mililitiro yamazi.

Ubwoko bwa pipeti nibice byibanze bya pipeti

Pipettes mubisanzwe ni sterile imwe-ikoreshwa ya pulasitike; zirashobora kandi kuba autoclavable, yongeye gukoreshwa ibirahuri.

Imiyoboro yose ikoresha umuyoboro iyo ucometse.

Umuyoboro ukuraho abashakashatsi gukenera umunwa nka mbere. Ubwo buryo bwo kuvoma bwa primitique ntabwo busabwa kuko bufite ubushobozi bwo guteza ingaruka zikomeye kumazi yinjizwa mumunwa.

Umupira wa pipette ni ubwoko bwa pipette nukuri neza. Mubisanzwe bihujwe nikirahuri cyikirahure kugirango cyohereze ibintu bitandukanye byamazi.

Pompe ya pompe nayo irakwiriye kubirahuri byikirahure, bishobora kwimura ubwinshi bwamazi yuzuye. Amapompo ya pipette arakwiriye mugutanga urugero rumwe rwamazi inshuro nyinshi.

Abafasha bungirije ni imiyoboro isanzwe. Igizwe n'ibice byinshi by'ingenzi: umunwa niho hashyizwemo pipeti n'ahantu hashyizwemo akayunguruzo, kurinda imbere umuyoboro wungirije udashobora kwanduza amazi.

Utubuto tubiri turashobora kuboneka kumaboko ya Assistant pipette. Iyo buto yo hejuru ikanda, amazi aba yifuzwa, kandi iyo buto yo hepfo ikanda, amazi arasohoka.

Imiyoboro myinshi yunganira nayo ifite ipfunwe ryo kugenzura igipimo cyamazi. Kurugero, irashobora gushyirwaho kugirango irekure amazi munsi yigitutu, cyangwa irashobora gushyirwaho kugirango irekure imbaraga zidafite imbaraga zo hanze.

Mugihe imiyoboro imwe yunganira izana umugozi w'amashanyarazi, inyinshi zikoreshwa na bateri.

Bamwe mu bafasha bungirije baza bafite igihagararo gihuye n'ahantu hafatirwa, bigatuma umufasha wungirije ashyirwa kuruhande rwayo mugihe adakoreshejwe adakuyeho umuyoboro.

Nkuko byavuzwe haruguru, umuyoboro umwe urashobora gukoresha ubunini butandukanye bwa pipeti bitewe nubunini bugomba gutwarwa, kuva kuri mililitiro 0.1 kugeza kuri mililitiro icumi.

Igikorwa cyibanze cya pipeti

Ubwa mbere, hitamo ubunini bukwiye bwa pipeti ukurikije ingano y'amazi ushaka kohereza. Noneho fungura paki uhereye hejuru, kora igice gusa hejuru yikimenyetso, winjize mumutwe wa pipette, hanyuma ukureho pake isigaye.

Ibikurikira, fata pipette ukoresheje ukuboko kumwe hanyuma ufungure umupfundikizo wa kontineri irimo amazi ushaka kwifuza. Komeza umuyoboro uhagaze neza, kanda buhoro buhoro buto yo hejuru kugirango wifuze buhoro buhoro icyitegererezo cyawe.

Koresha umurongo urangije kurukuta rwa pipette kugirango upime ingano y'amazi ushaka kohereza. Menya ko amajwi agomba gusomwa hepfo ya menisk, ntabwo ari hejuru.

Noneho urekure witonze amazi mubikoresho wahisemo, witondere kutareka umuyoboro wa pipette ukoraho hejuru yubusa.

Koresha ubwitonzi n'imbaraga zoroheje mugihe wirukanye amazi, cyane cyane mugihe ukoresha imiyoboro ntoya yubushobozi buke, kugirango wirinde kwanduza umufasha wa pipeti na sample, cyangwa kwangiza umufasha wungirije. Mishandling mugihe ukoresheje umufasha wa pipette birashobora kurakaza abandi bantu bafite uburambe muri laboratoire, bashobora gutandukanya umuyoboro kugirango basane. Iyo kuvoma amazi menshi cyangwa gusohora amazi, umuvuduko wo kohereza amazi urashobora kwiyongera mugukanda buto cyane.

Hanyuma, wibuke guta neza ibyatsi nyuma yo kohereza amazi.

Gusaba

Noneho ko uzi gukora pipette, reka turebe muburyo burambuye kuri laboratoire isanzwe.

Intambwe yingenzi mugihe cyo gutezimbere no gusya ingirabuzimafatizo ni ugukwirakwiza ingirabuzimafatizo mugisubizo cyanyuma. Guhagarika ingirabuzimafatizo birashobora kuvangwa buhoro kandi neza ukoresheje pipeti, icyarimwe ivanga ibisubizo byimiti na reagent.

Nyuma yo kwigunga cyangwa gutunganya ingirabuzimafatizo zigeragezwa, pipettes zirashobora gukoreshwa mu kwimura clone zose zo kwaguka cyangwa gusesengura ubushakashatsi.